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MB Sample ID: SA343042
Local Sample ID: | old_1 |
Subject ID: | SU003287 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Young donors (aged 25 years or younger) and older donors (aged 50 years or older). |
Gender: | Male and female |
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Combined analysis:
Analysis ID | AN005198 | AN005199 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Waters HPLC C18 (100 x 2.1mm, 1.7um) | Waters HPLC C18 (100 x 2.1mm, 1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | pmoles/mg tissue | nanomoles/mg of tissue |
Chromatography:
Chromatography ID: | CH003933 |
Chromatography Summary: | A ThermoFisher Q-Exactive HF-X mass spectrometer coupled to a Vanquish HPLC with a Waters Acquity UPLC BEH C18 (100 x 2.1 mm, 1.7 um) column was used for LC-MS/MS, with minor modifications to our previously-reported method. HPLC solvent A was 10 mM ammonium formate, 0.1% formic acid in acetonitrile:water (60:40), and solvent B was 10 mM ammonium formate, 0.1% formic acid in isopropanol:acetonitrile (90:10). A 27 min binary gradient at 0.28 mL/min was used: 0 min, 80:20 A/B; 3 min, 80:20 A/B; 5.5 min, 55:45 A/B; 8 min, 35:65 A/B; 13 min, 15:85 A/B; 14 min, 0:100 A/B; 20 min, 0:100 A/B; 20.2 min, 70:30 A/B; 27 min, 70:30 A/B. |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters HPLC C18 (100 x 2.1mm, 1.7um) |
Column Temperature: | 32 |
Flow Gradient: | 0 min, 80:20 A/B; 3 min, 80:20 A/B; 5.5 min, 55:45 A/B; 8 min, 35:65 A/B; 13 min, 15:85 A/B; 14 min, 0:100 A/B; 20 min, 0:100 A/B; 20.2 min, 70:30 A/B; 27 min, 70:30 A/B |
Flow Rate: | 0.28 mL/min |
Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |