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MB Sample ID: SA343179
Local Sample ID: | Old Leaf_Pyrus communis_OLR3 |
Subject ID: | SU003292 |
Subject Type: | Plant |
Subject Species: | Malus domestica;Pyrus communis;Apple-pear intergeneric hybrid |
Taxonomy ID: | 3750;23211 |
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Combined analysis:
Analysis ID | AN005207 | AN005208 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Waters Xevo TQ-XS | Waters Xevo TQ-XS |
Ion Mode | POSITIVE | NEGATIVE |
Units | mg/L | mg/L |
Chromatography:
Chromatography ID: | CH003940 |
Chromatography Summary: | Ultraperformance liquid chromatography was performed on a Waters Acquity UPLC system (Milford, MA) consisting of a binary pump, an online vacuum degasser, an autosampler, and a column compartment. Separation of the phenolic compounds was achieved on a Waters Acquity HSS T3 column 1.8 μm, 100 mm × 2.1 mm (Milford, MA, USA), kept at 40 °C. Mobile phase A was water containing 0.1% formic acid; mobile phase B was acetonitrile containing 0.1% formic acid. The flow was 0.4 mL/min, and the gradient profile was 0-0.1 min, 5% B; from 0 to 3 min, linear gradient to 20% B; from 3 to 4.3 min, isocratic 20% B; from 4.3 to 9 min, linear gradient to 45% B; from 9 to 11 min, linear gradient to 100% B; from 11 to 13 min, wash at 100% B; from 13.01 to 15 min, back to the initial conditions of 5% B. The injection volume of both the standard solutions and the samples was 2 μL. After each injection, the needle was rinsed with 600 μL of weak wash solution (water/methanol, 90:10) and 200 μL of strong wash solution (methanol/water, 90:10). Samples were kept at 6 °C during the analysis. |
Instrument Name: | Waters Acquity |
Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 40 |
Flow Gradient: | 0-0.1 min: 5% B, 0.1-3.0 min: linear 20%B, 3.0-4.3 min: isocratic 20% B, 4.3-9.0 min: linear 45% B, 9.0-11.0 min: linear 100% B, 11.0-13.0 min: wash 100% B, 13.01 – 15.0 min: back to initial 5% B |
Flow Rate: | 0.4 ml/min |
Solvent A: | 99.9% water/0.1% formic acid |
Solvent B: | 99.9% acetonitrile/0.1% formic acid |
Chromatography Type: | Reversed phase |