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MB Sample ID: SA346794
Local Sample ID: | DS2-039-031 |
Subject ID: | SU003304 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005231 | AN005232 | AN005233 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE | POSITIVE | NEGATIVE |
Units | peak area | peak area | peak area |
Chromatography:
Chromatography ID: | CH003958 |
Chromatography Summary: | Negative C18 global lipidomics |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 0-3 min 10-95% B at 0.3 mL/min, 3-4.2 min hold at 95% B at 0.3 mL/min, 4.2-4.3 min drop to 10% B at 0.45 mL/min, 4.3-4.9 min hold at 10%B while lowering flow rate to 0.4 mL/min, 4.9-5 min hold at 10%B while lowering flow rate to 0.3 mL/min. |
Flow Rate: | 0.3-0.4 ml/min |
Sample Injection: | 10 uL |
Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
Solvent B: | 90% isopropanol/10% acetonitrile; 5 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003959 |
Chromatography Summary: | Positive C18 global lipidomics |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 0-3 min 30-100% B at 0.3 mL/min, 3-4.2 min hold at 100% B at 0.3 mL/min, 4.2-4.3 min 100-30% B at 0.4 mL/min, 4.3-4.9 min hold at 30%B and 0.4 mL/min, 4.9-5 min hold at 30%B while lowering flow rate to 0.3 mL/min |
Flow Rate: | 0.3-0.4 ml/min |
Sample Injection: | 10 uL |
Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
Solvent B: | 90% isopropanol/10% acetonitrile; 5 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003960 |
Chromatography Summary: | Negative C18 fatty acids |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
Column Temperature: | 45 |
Flow Gradient: | 0-1 min 25% B and 0.3 ml/min; 1-2 min 25-50% B and 0.3 mL/min; 2–8 min 50–90% B and 0.3 mL/min; 8–10 min 90–99% B and 0.3 mL/min; 10–14 min hold at 99% B and 0.3 mL/min; 14–14.1 min 99–25% B and 0.3 mL/min; 14.1–16.9 min hold at 25% B and 0.4 mL/min; 16.9–17 min hold at 25% B and resume flow of 0.3 mL/min |
Flow Rate: | 0.3-0.4 ml/min |
Solvent A: | 75% water/25% acetonitrile; 5 mM ammonium acetate |
Solvent B: | 50% isopropanol/45% acetonitrile/5% water; 5 mM ammonium acetate |
Chromatography Type: | Reversed phase |