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MB Sample ID: SA351649
Local Sample ID: | 2729-1 |
Subject ID: | SU003330 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005266 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GC-2030 |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | Triple quadrupole |
MS instrument name | Shimadzu TQ8050NX |
Ion Mode | POSITIVE |
Units | Relative abundance |
Chromatography:
Chromatography ID: | CH003985 |
Chromatography Summary: | The GC-MS system used comprised of an AOC6000 autosampler, a 2030 Shimadzu gas chromatograph and a TQ8050NX triple quadrupole mass spectrometer (Shimadzu, Japan)with an electron ionisation source(-70eV). The mass spectrometer was tuned according to the manufacturer’s recommendations using tris-(perfluorobutyl)-amine (CF43). GC-MS was performed on a 30m Agilent DB-5 column with 0.25mm internal diameter column and 1µm film thickness. The injection temperature (inlet) was set at 280°C, the MS transfer line at 280°C and the ion source adjusted to 200°C. Helium was used as the carrier gas at a flow rate of 1 mL/min and argon gas was used in the collision cell to generate the MRM product ion. The analysis of the derivatised samples was performed under the following oven temperature program; 100°C start temperature, hold for 4 minutes, followed by a 10°C/min oven temperature ramp to 320°C with a following final hold for 11 minutes. |
Instrument Name: | Shimadzu GC-2030 |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Column Temperature: | 100 - 320 |
Flow Gradient: | N/A |
Flow Rate: | 1 mL/min |
Solvent A: | N/A |
Solvent B: | N/A |
Chromatography Type: | GC |