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MB Sample ID: SA352336
Local Sample ID: | Sham_Chiasm_2 |
Subject ID: | SU003344 |
Subject Type: | Amphibian |
Subject Species: | Xenopus laevis |
Taxonomy ID: | 8355 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005287 | AN005288 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Accucore C18 (150 x 2.1mm,2.6um) | Thermo Accucore C18 (150 x 2.1mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH003999 |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore C18 (150 x 2.1mm,2.6um) |
Column Temperature: | 55 |
Flow Gradient: | The gradient began at 10% B for 1 min, then shifted to 30% B for 1.5min, 50% for 3.5min, 60% for 10min, 80% for 2 min, 95% for 2 min, then stayed at 100% B for 6 min before ramping down to 10% B for 2 min. |
Flow Rate: | 260 ul/min |
Solvent A: | 50% acetonitrile/50% water; 0.1% formic acid; 5mM ammonium formate |
Solvent B: | 88% isopropanol/10% acetonitrile/2% water; 0.1% formic acid; 5mM ammonium formate |
Chromatography Type: | Reversed phase |