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MB Sample ID: SA353352
Local Sample ID: | Kidney_CreERT2_210 |
Subject ID: | SU003361 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005311 | AN005312 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um) | Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized to the median value of total lipid signals | Normalized to the median value of total lipid signals |
Chromatography:
Chromatography ID: | CH004016 |
Instrument Name: | Thermo Vanquish |
Column Name: | Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um) |
Column Temperature: | 50 |
Flow Gradient: | 0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B. |
Flow Rate: | 0.400 mL/min |
Solvent A: | 100% water; 0.1% formic acid; 10mM ammonium acetate |
Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate |
Chromatography Type: | Reversed phase |