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MB Sample ID: SA353374

Local Sample ID:38-08-18-2022-POS-Isaac-S14
Subject ID:SU003362
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

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Combined analysis:

Analysis ID AN005313
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Normalized to the median value of total lipid signals

Chromatography:

Chromatography ID:CH004017
Instrument Name:Thermo Vanquish
Column Name:Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um)
Column Temperature:50
Flow Gradient:0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B
Flow Rate:0.400 mL/min
Solvent A:100% water; 0.1% formic acid; 10mM ammonium acetate
Solvent B:50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate
Chromatography Type:Reversed phase
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