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MB Sample ID: SA354169
Local Sample ID: | 210309_MDA_Timecourse_RSL3_n3_4h_RSL3_1uM_29 |
Subject ID: | SU003381 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005345 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity H-Class |
Column | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex 6500+ |
Ion Mode | NEGATIVE |
Units | absolute intensities |
Chromatography:
Chromatography ID: | CH004047 |
Chromatography Summary: | Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 130 Å, 2.1×100 mm, Waters, Milford, MA) using an ExionLC UHPLC system. |
Instrument Name: | Waters Acquity H-Class |
Column Name: | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
Column Temperature: | 45°C |
Flow Gradient: | The gradient was ramped from 75 to 85% B over 5 min and further increased to 100% B within 2 min, followed by isocratic elution for another 2 min. |
Flow Rate: | 0.75 ml/min |
Solvent A: | water/acetonitrile 90/10, 2 mM ammonium acetate |
Solvent B: | water/acetonitrile 5/95, 2 mM ammonium acetate |
Chromatography Type: | Reversed phase |