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MB Sample ID: SA354492

Local Sample ID:LMRI_DietRev_plasma_20B4
Subject ID:SU003393
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:12-16 months
Weight Or Weight Range:25-45 g
Gender:Male
Animal Animal Supplier:The Scripps Research Institute Vivarium
Animal Housing:5/cage
Animal Light Cycle:12h dark/12 h light
Animal Feed:Special diets; see group labeling
Animal Water:Ad libitum

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Combined analysis:

Analysis ID AN005359
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6460
Column Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6460 QQQ
Ion Mode POSITIVE
Units Normalized ion count/L

Chromatography:

Chromatography ID:CH004060
Chromatography Summary:The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B.
Instrument Name:Agilent 6460
Column Name:Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm)
Column Temperature:40℃
Flow Gradient:0 min, 82% B; 3 min, 82% B; 4 min, 90% B; 18 min, 99% B; 25 min, 99% B; 27 min, 82% B; and 30 min, 82% B
Flow Rate:0.5 mL/min
Solvent A:100% water; 0.2% formic acid; 2 mM ammonium formate
Solvent B:100% methanol; 0.2% formic acid; 1 mM ammonium formate
Chromatography Type:Reversed phase
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