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MB Sample ID: SA354804
| Local Sample ID: | CTL_12dpi_Ret1_POS1 |
| Subject ID: | SU003398 |
| Subject Type: | Amphibian |
| Subject Species: | Xenopus laevis |
| Taxonomy ID: | 8355 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005369 | AN005370 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH004068 |
| Chromatography Summary: | Positive ion mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
| Column Temperature: | 35 C |
| Flow Gradient: | The gradient began at 1.0% B for 1 min, then shifted to 95.0% B for 9 minutes, then stayed at 95.0% B for 1 min before ramping down quickly to 1.0% B and held for 5 minutes. |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 95% acetonitrile/5% water; 10mM Ammonium Formate; 0.1% formic acid |
| Solvent B: | 50% acetonitrile/50% water; 10mM Ammonium Formate; 0.1% formic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH004069 |
| Chromatography Summary: | Negative ion mode |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
| Column Temperature: | 35 C |
| Flow Gradient: | The gradient began at 1.0% B for 1 min, then shifted to 95.0% B for 9 minutes, then stayed at 95.0% B for 1 min before ramping down quickly to 1.0% B and held for 5 minutes. |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 95% acetonitrile/5% water; 10mM Ammonium Acetate; 0.1% acetic acid |
| Solvent B: | 50% acetonitrile/50% water; 10mM Ammonium Acetate; 0.1% acetic acid |
| Chromatography Type: | HILIC |
