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MB Sample ID: SA354980
Local Sample ID: | Left_Tect_SHAM1_NEG3 |
Subject ID: | SU003399 |
Subject Type: | Amphibian |
Subject Species: | Xenopus laevis |
Taxonomy ID: | 8355 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005371 | AN005372 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH004070 |
Chromatography Summary: | Positive ion mode |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
Column Temperature: | 35 C |
Flow Gradient: | The gradient began at 1.0% B for 1 min, then shifted to 95.0% B for 9 minutes, then stayed at 95.0% B for 1 min before ramping down quickly to 1.0% B for 5 minutes. |
Flow Rate: | 0.5 ml/min |
Solvent A: | 95% acetonitrile/5% water/10mM Ammonium Formate/0.1% formic acid; 95% acetonitrile/5% water/10mM Ammonium Acetate/0.1% acetic acid |
Solvent B: | 50% acetonitrile/50% water/10mM Ammonium Formate/0.1% formic acid |
Chromatography Type: | HILIC |
Chromatography ID: | CH004071 |
Chromatography Summary: | Negative ion mode |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore Amide HILIC (150 x 2.1mm, 2.6um) |
Column Temperature: | 35 C |
Flow Gradient: | The gradient began at 1.0% B for 1 min, then shifted to 95.0% B for 9 minutes, then stayed at 95.0% B for 1 min before ramping down quickly to 1.0% B for 5 minutes. |
Flow Rate: | 0.5 ml/min |
Solvent A: | 95% acetonitrile/5% water; 10mM Ammonium Acetate; 0.1% acetic acid |
Solvent B: | 50% acetonitrile/50% water; 10mM Ammonium Acetate; 0.1% acetic acid |
Chromatography Type: | HILIC |