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MB Sample ID: SA355160
| Local Sample ID: | H99-iron-20Pi-6h_B |
| Subject ID: | SU003401 |
| Subject Type: | Fungi |
| Subject Species: | Cryptococcus neoformans |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005375 | AN005376 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Bruker Impact II | Bruker Impact II |
| Column | Agilent InfinityLab Poroshell 120 HILIC-Z column (2.7 µm particle size, 150 x 2.1 mm) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact HD | Bruker Impact HD |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | Rel Abundance | Rel Abundance |
Chromatography:
| Chromatography ID: | CH004074 |
| Chromatography Summary: | A novel method optimized for highly polar compounds was used to analyze samples using Hydrophilic Interaction Liquid Chromatography (HILIC) |
| Instrument Name: | Bruker Impact II |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z column (2.7 µm particle size, 150 x 2.1 mm) |
| Column Temperature: | 30 |
| Flow Gradient: | the initial condition of 90% B was held for 2 minutes, followed by a linear gradient to 40% B over 6 minutes, then maintained at 40% B for 2 minutes, returned to 90% B over 1.1 minutes, and equilibrated for 4.9 minutes, resulting in a total run time of 16 minutes |
| Flow Rate: | 0.3 ml/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium acetate; 5 µM medronic acid |
| Solvent B: | 90% acetonitrile/10% water ; 0.1% formic acid; 10 mM ammonium acetate; 5 µM medronic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH004075 |
| Chromatography Summary: | Separation of compounds was achieved using a multigradient method on an Acquity CSH |
| Instrument Name: | Bruker Impact II |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | 0 min 15% B; 0–2 min 30% B; 2–2.5 min 50% B; 2.5–12 min 80% B; 12–12.5 min 99% B; 12.5–13.5 min 99% B; 13.5–13.7 min 15% B; 13.7-17 min 15% B. |
| Flow Rate: | 0.5 ml/min |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
