Summary of Study ST000040
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000040 |
Study Title | Heatshock response of C. elegans using IROA (I) |
Institute | University of Florida |
Department | Biochemistry & Molecular Biology |
Laboratory | Edison |
Last Name | Stupp |
First Name | Gregory |
stuppie@ufl.edu | |
Submit Date | 2013-12-10 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 3.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2014-03-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000060 | AN000061 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Accela 1250 | Thermo Accela 1250 |
Column | Thermo Scientific Gold aQ | Thermo Scientific Gold aQ |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000041 |
Chromatography Summary: | Three microliters of each sample were injected onto a Thermo Scientific Gold aQ (150 × 2.1 mm, 1.9 μm) column using a column temperature of 40 °C and a flow rate of 600 μL/min with a gradient of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) from 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition. The inlet to the Orbitrap was held at a temperature of 320 °C, and the S-lens RF Level was set to 35%. The FR resolution was set to 70 000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm, externally calibrated. |
Instrument Name: | Thermo Accela 1250 |
Column Name: | Thermo Scientific Gold aQ |
Column Temperature: | 40 °C |
Flow Gradient: | A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) from 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition |
Sample Injection: | 3 ?L |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 18.5 min |
Chromatography Type: | Reversed phase |