Summary of Study ST000293
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000234. The data can be accessed directly via it's Project DOI: 10.21228/M8DG6Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000293 |
Study Title | Vitamin B6 Effects on one-carbon metabolism |
Study Type | Before-and-after |
Study Summary | Vitamin B6 supplementation with 10 mg/d pyridoxine-HCl for 28-d was given to oral contraceptive (OC) users who initially had vitamin B6 deficiency (PLP < 30 nmol/L). Samples are analyzed before and after supplementation. In addition samples from OC users with low (PLP < 30 nmol/L) , middle (PLP 31-99 nmol/L) and high (PLP > 100 nmol/L) vitamin B6 concentration are compared. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Food Science and Human Nutrition |
Last Name | Gregory |
First Name | Jesse |
Address | -- |
jfgy@ufl.edu | |
Phone | 352-392-1991 ext 225 |
Submit Date | 2015-03-13 |
Num Groups | 3 |
Total Subjects | 72 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000468 | AN000469 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 |
Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Thermo Quantiva QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | uM | uM |
Chromatography:
Chromatography ID: | CH000332 |
Chromatography Summary: | Waters AccQ·Tag Amino Acid Assay |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000333 |
Methods Filename: | SB_OA_Assay.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 45 |
Flow Rate: | 0.3 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |