Summary of Study ST000326
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000256. The data can be accessed directly via it's Project DOI: 10.21228/M8PS35 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000326 |
Study Title | Role of medium in bacterial growth (HILIC chromatography) |
Study Summary | Experiment to test how different growth mediums affect bacterial growth. The supernatants of 17 strains of bacteria (10 grown in one medium and 7 grown in another medium) were submitted for metabolite analysis. |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2014-11-25 |
Num Groups | 4 |
Total Subjects | 38 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-01-27 |
Release Version | 2 |
Release Comments | Updated study design factors |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000519 | AN000520 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Unspecified | Unspecified |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000364 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_HILIC_QTOF_MS.pdf |
Instrument Name: | Unspecified |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Pressure: | 200-700 bar |
Column Temperature: | 45 C |
Flow Gradient: | 100% B to 30% B |
Flow Rate: | 0.4 mL/min |
Internal Standard: | See data dictionary |
Retention Time: | See data dictionary |
Sample Injection: | 3 uL |
Solvent A: | 100% water; 0.125% formic acid; 10 mM ammonium formate, pH 3 |
Solvent B: | 95% acetonitrile/5% water; 0.125% formic acid; 10 mM ammonium formate, pH 3 |
Analytical Time: | 14 min |
Capillary Voltage: | 4500 |
Time Program: | 16.75 min |
Weak Wash Solvent Name: | 1:1 ACN:H2O |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | 1:1 ACN:H2O |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | HILIC |