Summary of Study ST000456
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000352. The data can be accessed directly via it's Project DOI: 10.21228/M8JK5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000456 |
Study Title | Metabolomic changes during active immunization with anti-METH vaccines. |
Study Summary | A targeted metabolomics analysis was performed on serum samples from rats immunized with a methamphetamine-like hapten covalently bound to keyhole limpet hemocyanin (KLH) monomers, and controls. The subjects were approximately 300 g adult male Sprague Dawley rats. RTI International RCMRC received pre-immune serum from RI 11-03A (n=12) which is a matched control serum that was collected before starting immunizations, and week 17 bleeds from RI 11-03A (n=12), RI 11-03B (n=8), and RI 11-03C (n=12). The A-C nomenclature denotes A) complete antigen with adjuvant, B) complete antigen without adjuvant and C) only KLH carrier protein and adjuvant without a METH-like hapten. The rats’ serum samples were extracted and prepared for a combined flow injection (FIA) and LC-MS/MS assay (Biocrates AbsoluteIDQ® p180 kit). The Biocrates AbsoluteIDQ® p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) can quantitatively measure ~188 biologically relevant metabolites from five analyte groups (acylcarnitines, amino acids, biogenic amines, glycerophospho- and sphingolipids, and hexoses) on a triple quadrupole mass spectrometer. |
Institute | University of North Carolina |
Department | Discovery-Sciences-Technology (DST) |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2016-08-31 |
Num Groups | 4 |
Total Subjects | 44 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000713 | AN000714 | AN000715 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1100 | Agilent 1100 | Agilent 1100 |
Column | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | Intensity | Intensity | Intensity |
Chromatography:
Chromatography ID: | CH000514 |
Instrument Name: | Agilent 1100 |
Column Name: | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) |
Column Temperature: | 50 degrees C |
Flow Rate: | 0.5mL/min |
Chromatography Type: | Reversed phase |