Summary of Study ST000470
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000361. The data can be accessed directly via it's Project DOI: 10.21228/M8CW21 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000470 |
Study Title | Exahustive degradation of nucleotide triphosphates |
Study Type | Comparison of degradation kinetics |
Study Summary | The degradation kinetics of nucleotide triphosphates (ATP, GTP, UTP and CTP) were evaluated under boiling ethanol extraction conditions (95°C) during 0 to 300 minutes. |
Institute | University of Groningen |
Department | Analytical Biochemistry |
Last Name | Bischoff |
First Name | Rainer |
Address | Antonius Deusinglaan 1 (XB20), Building 3226, room 601, 9713 AV Groningen, The Netherlands |
r.p.h.bischoff@rug.nl | |
Phone | NA |
Submit Date | 2016-09-07 |
Publications | Gil, A., Siegel, D., Bonsing-Vedelaar, S. et al. The degradation of nucleotide triphosphates extracted under boiling ethanol conditions is prevented by the yeast cellular matrix. Metabolomics (2017) 13:1. doi:10.1007/s11306-016-1140-4 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2018-04-10 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN000734 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity |
Column | Phenomenex Luna NH2 (100 x 2.0mm,3um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt-G2-Si |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000527 |
Chromatography Summary: | Untargeted HILIC Method |
Instrument Name: | Waters Acquity |
Column Name: | Phenomenex Luna NH2 (100 x 2.0mm,3um) |
Column Temperature: | 20C |
Flow Gradient: | 30% eluent A to 99% eluent A in 8 min, followed by isocratic elution at 99% eluent A until 14 min. A conditioning cycle of 6 min with the initial proportions of eluents A and B was performed prior to the next analysis. |
Flow Rate: | 0.25 mL/min |
Sample Injection: | 10 µL |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Analytical Time: | 20 min |
Chromatography Type: | HILIC |