Summary of Study ST000586
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000429. The data can be accessed directly via it's Project DOI: 10.21228/M8M30H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000586 |
Study Title | Evaluation of specific concentrations for use in experimental protocol |
Study Type | GC-MS non-targeted metabolomic profiling |
Study Summary | This study evaluated specific plasma concentrations and compared the optimal plasma extract volume established in the first study (Effects of dilution on analyte identification and quantification) with the volume previously used in the current institutional protocol. The findings of this study lead to recommendations for experimental design in GC-MS-based metabolomic profiling of human plasma. |
Institute | Duke University |
Department | Duke Molecular Physiology Institute |
Last Name | Wang |
First Name | Hanghang |
Address | 300 North Duke Street, Durham, NC, 27701, USA |
hanghang.wang@duke.edu | |
Phone | +1 919 884 0025 |
Submit Date | 2017-04-11 |
Num Groups | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2018-06-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000901 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Agilent DB5-MS (15m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975B |
Ion Mode | POSITIVE |
Units | Peak area (Log2 transformed) |
Chromatography:
Chromatography ID: | CH000641 |
Chromatography Summary: | GC/MS methods follow previous studies using a 6890 N GC connected to a 5975B Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122–5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
Instrument Name: | Agilent 6890N |
Column Name: | Agilent DB5-MS (15m x 0.25mm,0.25um) |
Flow Rate: | 2.0 mL/min |
Time Program: | initial GC oven temperature of 60°C increased at 10°C/min to a final temperature of 325°C |
Chromatography Type: | GC |