Summary of Study ST000591
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000591 |
Study Title | Metablomic profiling in acc1-5 mutant and wild type arabidiopsis |
Study Summary | This experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites. |
Institute | Agriculture and Agri-Food Canada |
Department | London Research and Development Centre |
Laboratory | Renaud |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford street, London, Ontario, Canada |
justin.renaud@agr.gc.ca | |
Phone | 519-953-6698 |
Submit Date | 2017-03-12 |
Publications | Chen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1. |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-25 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN000906 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 |
Column | SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000643 |
Chromatography Summary: | Samples were resolved using HILIC column |
Instrument Name: | Agilent 1290 |
Column Name: | SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um) |
Column Temperature: | 30C |
Flow Gradient: | 87% B for 5 minutes, decreased to 55% over 8 minutes and held for 4 minutes before returning to 87% over 3 minute |
Flow Rate: | 0.3 ml min-1 |
Internal Standard: | 13C6 phenylalanine internal standard |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 4 |
Solvent B: | 90% acetonitrile/10% water; 0.1% formic acid |
Chromatography Type: | HILIC |