Summary of Study ST001167
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000779. The data can be accessed directly via it's Project DOI: 10.21228/M8097N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001167 |
Study Title | Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry |
Study Summary | We developed a stable-isotope tracing capillary electrophoresis (CE)-MS metabolomics approach to cover polar metabolites as well as isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multi-dimensional data. The practicability is demonstrated analysing 13C-U-glucose exposed prostate cancer and non-cancer cells. |
Institute | Dalian Institute Of Chemical Physics |
Last Name | Wang |
First Name | Zhichao |
Address | 457, Zhongshan Road |
wangzc05@dicp.ac.cn | |
Phone | +86-15998625250 |
Submit Date | 2019-01-06 |
Study Comments | Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry - A Novel Tool Complementing Metabolomics Analyses of Polar Metabolites |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001929 | AN001930 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
Column | a fused silica capillary (50 μm i.d. × 80 cm) | a fused silica capillary (50 μm i.d. × 80 cm) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative intensity after normalization | Relative intensity after normalization |
Chromatography:
Chromatography ID: | CH001400 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | a fused silica capillary (50 μm i.d. × 80 cm) |
Column Temperature: | 20 |
Flow Rate: | a positive voltage of 27 kV |
Injection Temperature: | 20 |
Solvent A: | 1 M formic acid |
Chromatography Type: | CE |
Chromatography ID: | CH001401 |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | a fused silica capillary (50 μm i.d. × 80 cm) |
Column Temperature: | 20 |
Flow Rate: | internal pressure of 15 mbar and positive voltage of 30 kV |
Injection Temperature: | 20 |
Solvent A: | 100% water; 25 mM ammonium acetate; 75 mM ammonium phosphate, pH 8.5 |
Chromatography Type: | CE |