Summary of Study ST001298
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000879. The data can be accessed directly via it's Project DOI: 10.21228/M82M47 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001298 |
Study Title | Metabolome Profiling of Synechococcus elongatus PCC 11801 strains engineered for Succinate Production |
Study Type | Measurement of relative metabolite pools of wild type and engineered Synechococcus elongatus PCC 11801 strains using Isotopic Ratio Method |
Study Summary | Experiments to measure relative metabolite pools of wild type Synechococcus elongatus PCC 11801 and its recombinants producing succinate. The wild type and the engineered strains producing succinate were cultivated at 1% CO2 and their metabolome data was collected in three biological and three technical replicates (n=9). The study aims to find metabolomics changes between the wild type and the engineered to identify potential rate-limiting steps that be used as targets for improved production. |
Institute | Indian Institute of Technology Bombay |
Department | Department of Chemical Engineering |
Laboratory | Bio systems Engineering Lab |
Last Name | Wangikar |
First Name | Pramod P |
Address | Department of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India - 400076 |
wangikar@iitb.ac.in | |
Phone | 22254215 |
Submit Date | 2020-01-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2020-07-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002162 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Area Ratio |
Chromatography:
Chromatography ID: | CH001580 |
Instrument Name: | Shimadzu 20AD |
Column Name: | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
Flow Gradient: | The gradient method used is as follows: 0% B (0.01 min), 0% B (2 min), 35% B (8 min), 35% B (10.5 min), 90% B (15.50 min), 90% B (20.5 min), 0% B (22 min), and 0% B (30 min) |
Flow Rate: | 0.3 mL/minute |
Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |