Summary of Study ST001320
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000897. The data can be accessed directly via it's Project DOI: 10.21228/M8R704 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001320 |
Study Title | Examination of labeled glucose utilization in central carbon metabolism in HeLa cells post WNT stimulation |
Study Type | In vitro |
Study Summary | HeLa cells were treated with media containing either vehicle or WNT in combination with UC13-glucose for 20 min or 60 min. |
Institute | University of California, Los Angeles |
Department | Biological Chemisty |
Laboratory | Christofk Lab |
Last Name | Christofk |
First Name | Heather |
Address | 615 Charles E Young Drive South Los Angeles, CA 90095 |
hchristofk@mednet.ucla.edu | |
Phone | (310) 794-4248 |
Submit Date | 2020-02-19 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2020-07-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002196 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | MS1 AUC integration |
Chromatography:
Chromatography ID: | CH001610 |
Chromatography Summary: | Samples were run on a Vanquish (Thermo Scientific) UHPLC system with mobile phase A (5mM NH4AcO, pH 9.9) and mobile phase B (ACN) at a flow rate of 200 µL/min on a Luna 3 um NH2 100A (150 × 2.0mm) at 40°C with a gradient going from 15% A to 95% A in 18 min followed by an 11 minute isocratic step. |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Column Temperature: | 40 |
Flow Gradient: | 15% A to 95% A in 18 min followed by an 11 minute isocratic step |
Flow Rate: | 200 µL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |