Summary of Study ST001765
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001765 |
Study Title | Optimization of redox metabolite detection in mammalian cells (part I) |
Study Summary | Conditions were tested to optimize number of cells and extraction buffer for the detection of redox reactive metabolites from mammalian cells. Four different extraction buffers were compared. Derivatization of glutathione was explored as a condition as well. This is an independent repeat. |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | Pathology |
Laboratory | Naama Kanarek |
Last Name | Petrova |
First Name | Boryana |
Address | 300 Longwood Av, Boston, MA, 2115, USA |
boryana.petrova@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2021-04-21 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002872 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | EMD Millipore ZIC HILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | ppm |
Chromatography:
Chromatography ID: | CH002127 |
Chromatography Summary: | ZIC-pHILIC chromatography: One ml of reconstituted sample was injected into a ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 20 mM ammonium carbonate, 0.1% ammonium hydroxide. Gradient conditions were: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. The column oven and autosampler tray were held at 25 °C and 4 °C, respectively. |
Instrument Name: | Thermo Vanquish |
Column Name: | EMD Millipore ZIC HILIC (150 x 2.1mm,5um) |
Column Temperature: | 25 |
Flow Gradient: | linear gradient from 20% to 80% B; 20-20.5 min: from 80% to 20% B; 20.5-28 min: hold at 20% B. |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide |
Chromatography Type: | HILIC |