Summary of Study ST001773
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001773 |
Study Title | Application of the redox metabolite detection method for mouse biofluids (part II) |
Study Summary | This study was aimed at optimizing redox metabolites detection from mammalian biofluids. Three different extraction conditions were compared, including derivatization of glutathione. This study was with mouse plasma. |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | Pathology |
Laboratory | Naama Kanarek |
Last Name | Petrova |
First Name | Boryana |
Address | 300 Longwood Av, Boston, MA, 2115, USA |
boryana.petrova@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2021-04-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002880 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | EMD Millipore ZIC HILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | ppm |
Chromatography:
Chromatography ID: | CH002135 |
Chromatography Summary: | ZIC-pHILIC chromatography: One ml of reconstituted sample was injected into a ZIC-pHILIC 150 × 2.1 mm (5 µm particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 20 mM ammonium carbonate, 0.1% ammonium hydroxide. Gradient conditions were: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. The column oven and autosampler tray were held at 25 °C and 4 °C, respectively. |
Instrument Name: | Thermo Vanquish |
Column Name: | EMD Millipore ZIC HILIC (150 x 2.1mm,5um) |
Column Temperature: | 25 |
Flow Gradient: | linear gradient from 20% to 80% B; 20-20.5 min: from 80% to 20% B; 20.5-28 min: hold at 20% B. |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide |
Chromatography Type: | HILIC |