Summary of Study ST001794
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001138. The data can be accessed directly via it's Project DOI: 10.21228/M8M68K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001794 |
Study Title | Metabolomics Analysis of Time-Series Gastrointestinal Lumen Samples |
Study Summary | Samples were retrieved from a human small intestine samples over 8 hours. Metabolomics analysis followed resulting in many annotated metabolites. Intensity profiles gives insight into gastrointestinal functions. |
Institute | University of California, Davis |
Last Name | Folz |
First Name | Jacob |
Address | 451 Health Science Drive |
jfolz@ucdavis.edu | |
Phone | 7155636311 |
Submit Date | 2021-05-17 |
Total Subjects | 1 |
Num Males | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-06-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002911 | AN002912 | AN002913 | AN002914 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak Height Intensity | Peak Height Intensity | Peak Height Intensity | Peak Height Intensity |
Chromatography:
Chromatography ID: | CH002157 |
Chromatography Summary: | HILIC |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | The gradient started at 100% B, was held at 100% B from 0-2 minutes, decreased to 70% B between 2-7.7 minutes, decreased to 40% between 7.7 and 9.5 minutes, was held at 40% B from 9.5-12.75 minutes, returned to 100% B between 12.75-12.85 minutes, and was held at 100% B from 12.85-17 minutes. |
Flow Rate: | 0.4 ml/min |
Sample Injection: | 3 uL |
Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate |
Chromatography Type: | HILIC |
Chromatography ID: | CH002158 |
Chromatography Summary: | CSH |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 65 |
Flow Gradient: | The LC gradient started at 15% B, increased to 30% B from 0-2 minutes, increased to 48% B from 2-2.5 minutes, increased to 82% B from 2.5-11 minutes, increased to 99% B from 11-11.5 minutes, held at 99% B from 11.5-12 minutes, returned to 15% B from 12-12.1 minutes and held at 15% B from 12.1-15 minutes. |
Flow Rate: | 0.6 ml/min |
Sample Injection: | 4 uL |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate(+ mode); 40% water/60% acetonitrile; 10 mM ammonium acetate(- mode) |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate for positive mode ionization, and 10mM ammonium acetate for negative mode ionization. |
Chromatography Type: | Reversed phase |