Summary of Study ST001935
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001224. The data can be accessed directly via it's Project DOI: 10.21228/M8H12N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001935 |
Study Title | Metabolomic profiling of spontaneous macaque model for diabetes mellitus |
Study Summary | The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, insights into the mechanisms underlying its pathogenesis are urgently needed. We reported the multi-omics profiling of the liver and sera of both peripheral blood and hepatic portal vein blood from Macaca fascicularis with spontaneous diabetes mellitus with a chow diet. The other two groups of the monkeys fed with chow diet and high-sugar high-fat (HSHF) diet, respectively, were included for comparison. These multi-omics datasets can provide a comprehensive picture of the molecular changes caused by diabetes in primates. Analyses of various omics datasets revealed the alterations of high consistency. Correlation between transcripts and proteins derived from the same genes was observed across individuals for most genes, especially the ones of differential expression. As a result, we found that distinct patterns of the metabolome, proteome, and transcriptome between spontaneous diabetes and HSHF diet-induced diabetes compared with healthy individuals. |
Institute | Xiamen University |
Last Name | Yang |
First Name | Zhu |
Address | OEW901, Oen Hall Building West Wing,, Ho Sin Hang Campus, Hong Kong Baptist University |
yangzhu@gmail.com | |
Phone | (+852)34115162 |
Submit Date | 2021-09-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | GC-MS |
Release Date | 2022-02-19 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003145 | AN003146 | AN003147 | AN003148 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH002327 |
Chromatography Summary: | The analysis of hydrophilic metabolites used 0.1% formic acid (in water) and acetonitrile with 0.1% formic acid as mobile phases, with a gradient (V/V) as following: 95:5 at 0 min, 10:90 at 11.0 min, 10:90 at 12.0 min, 95:5 at 12.1 min, and 95:5 at 14.0 min. |
Instrument Name: | Shim-pack UFLC SHIMADZU CBM A system |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Flow Gradient: | 95:5 at 0 min, 10:90 at 11.0 min, 10:90 at 12.0 min, 95:5 at 12.1 min, and 95:5 at 14.0 min. |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002328 |
Chromatography Summary: | The two mobile phases for hydrophobic compounds were 0.04% acetic acid and acetonitrile with 0.04% acetic acid, and the gradient (V/V) program was 95:5 at 0 min, 5:95 at 11.0 min, 5:95 at 12.0 min, 95:5 at 12.1 min and 95:5 at 14.0 min. |
Instrument Name: | Shim-pack UFLC SHIMADZU CBM A system |
Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
Flow Gradient: | 95:5 at 0 min, 5:95 at 11.0 min, 5:95 at 12.0 min, 95:5 at 12.1 min and 95:5 at 14.0 min. |
Solvent A: | 100% water; 0.04% acetic acid |
Solvent B: | 100% acetonitrile; 0.04% acetic acid |
Chromatography Type: | Reversed phase |