Summary of Study ST002024
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001286. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002024 |
Study Title | Plasmodium falciparum stable-isotope carbon labeling to explore metabolic consequences of keto–acid dehydrogenase disruption |
Study Summary | Plasmodium falciparum cells in culture were treated with respective universally labelled carbon-13 metabolites for 2.5 hours at standard culture concentrations (glucose or glutamine) or 5 mM (acetate). Metabolites were isolated using 90% methanol, dried, reconstituted in HPLC-grade water, and analyzed by HPLC/MS. Resulting data were analyzed and compiled to generate study data. |
Institute | Pennsylvania State University |
Department | Chemistry |
Laboratory | Llinás Laboratory |
Last Name | Llinás |
First Name | Manuel |
Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
mul27@psu.edu | |
Phone | 814-867-3444 |
Submit Date | 2021-07-15 |
Num Groups | 16 |
Total Subjects | 146 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-10-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003294 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters XSelect HSS (100 x 2.1mm,2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | Peak Abundance (normalized, blank subtracted, and corrected for baseline noise) |
Chromatography:
Chromatography ID: | CH002433 |
Chromatography Summary: | Ion-pairing method using reverse-phase chromatography setup. |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters XSelect HSS (100 x 2.1mm,2.5um) |
Column Temperature: | 30 |
Flow Rate: | 0.200 mL/minute |
Solvent A: | 97% water/3% methanol; 15 mM acetic acid; 10 mM tributylamine; 2.5 uM medronic acid |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |