Summary of Study ST002053
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001297. The data can be accessed directly via it's Project DOI: 10.21228/M82H7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002053 |
Study Title | Resistance to NaFAc of in vitro maturated Toxoplasma gondii bradyzoites in human myotubes. |
Study Summary | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. These data characterize bradyzoites and tachyzoites treated with NaFAc. |
Institute | Robert Koch-Institute |
Last Name | Blume |
First Name | Martin |
Address | Seestr. 10, Berlin, Berlin, 13353, Germany |
blumem@rki.de | |
Phone | +49 30 18754 2572 |
Submit Date | 2022-01-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003343 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | UNSPECIFIED |
Units | counts |
Chromatography:
Chromatography ID: | CH002475 |
Chromatography Summary: | Chromatographic separation was achieved on a Vanquish Flex fitted with an ACQUITY UPLC BEH Amide column (Waters). Running a 25 min linear gradient starting with 90 % eluent A (10 mM ammonium carbonate in acetonitrile) / 10 % eluent B (10 mM ammonium carbonate in water) and ending with 40 % eluent A / 60 % eluent B, followed by washing and equilibration steps. |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Flow Gradient: | Running a 25 min linear gradient starting with 90% A / 10% B and ending with 40% eluent A / 60% eluent B, followed by washing and equilibration steps. |
Solvent A: | 100% acetonitrile; 10 mM ammonium carbonate |
Solvent B: | 100% water; 10 mM ammonium carbonate |
Chromatography Type: | HILIC |