Summary of Study ST002076
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001316. The data can be accessed directly via it's Project DOI: 10.21228/M8M990 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002076 |
Study Title | Metabolomic study of Escherichia coli K-12 MG1655 WT and its transcriptional regulator double mutants under anaerobic fermentation conditions |
Study Type | Metabolomics |
Study Summary | Metabolomic analysis of Wildtype, fnr_arcA (FA), arcA_ihf (AI) and fnr_ihf (FI) mutants in glucose minimal media under anaerobic fermentation conditions during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor anaerobically at 37 degrees Celsius and 150 rpm. This study aims to characterize and compare the metabolic profiles of all these strains. |
Institute | IIT Bombay |
Department | Department of Chemical Engineering |
Laboratory | Systems Biology and Metabolic Engineering Laboratory (SBMEL) |
Last Name | Pal |
First Name | Ankita |
Address | Department of Chemical Engineering, IIT Bombay, Mumbai, Maharashtra, 400076, India |
pal.ankita90@gmail.com | |
Phone | 08879536816 |
Submit Date | 2022-02-02 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-06-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003383 | AN003384 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | umol/gDCW and Height ratio/gDCW | umol/gDCW and Height ratio/gDCW |
Chromatography:
Chromatography ID: | CH002501 |
Chromatography Summary: | The detailed chromatography and MS protocol has been described in "Method_msi.pdf" file. |
Methods Filename: | Method_msi.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Chromatography Type: | HILIC |