Summary of Study ST002245
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001433. The data can be accessed directly via it's Project DOI: 10.21228/M8GX29 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002245 |
Study Title | Deciphering the metabolomic differences between two fast-growing cyanobacteria, S.elongatus PCC 11801 and 11802 via metabolite profiling |
Study Summary | The study aims to identify the metabolic differences between two promising fast-growing, non-model cyanobacterial strains, S. elongatus PCC 11801 and PCC 11802. To this end, experiments were carried out to measure metabolite levels in the two cyanobacterial strains grown in shake flasks at a similar light intensity of approx. 300-350 µmole photons.m-2. s-1. The samples for metabolomics analysis were collected during the exponential growth phase at an optical cell density of 0.5-0.6. Isotopic ratio method was utilized to compare the metabolite levels and delineate the differences in their metabolic pathways. |
Institute | Indian Institute of Technology Bombay |
Department | Chemical Engineering |
Last Name | Wangikar |
First Name | Pramod |
Address | Biosystems and Bioengineering Lab, Department of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India -400076 |
wangikar@iitb.ac.in | |
Phone | +91 22 2576 72 32 |
Submit Date | 2022-07-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003665 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | Triple TOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Area Ratios |
Chromatography:
Chromatography ID: | CH002715 |
Instrument Name: | Shimadzu 20AD |
Column Name: | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
Flow Gradient: | The gradient method used is as follows: 0% B (0.01 min), 0% B (2 min), 35% B (8 min), 35% B (10.5 min), 90% B (15.50 min), 90% B (20.5 min), 0% B (22 min), and 0% B (30 min) |
Flow Rate: | 0.3 mL/minute |
Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |