Summary of Study ST002399
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001533. The data can be accessed directly via it's Project DOI: 10.21228/M8J99C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002399 |
Study Title | Metabolomics of Adipocyte-Conditioned Media Compared to Stromal Cell- and Un-conditioned Media |
Study Type | Untargeted MS |
Study Summary | The metabolomics profiles of adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were analyzed by untargeted mass spectrometry. |
Institute | Emory University |
Department | Pediatrics |
Laboratory | Joshua Chandler, PhD |
Last Name | Chandler |
First Name | Joshua |
Address | 2015 Uppergate Drive NE, Atlanta, GA 30322 |
joshua.chandler@emory.edu | |
Phone | 404-727-3536 |
Submit Date | 2022-10-21 |
Num Groups | 3 (ACM vs SCM vs UCM) |
Total Subjects | 5 replicates of each group (15 total) |
Publications | submitted to JNCI |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-06-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003906 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH002892 |
Chromatography Summary: | A VanquishTM Horizon Binary ultrahigh performance liquid chromatography system coupled to a Q Exactive High Field Hybrid Orbitrap mass spectrometer (ThermoFisher) was used for data collection. A SeQuant® ZIC-HILIC column (Millipore Sigma, 3.5 µm, 100 Å, 150 x 2.1 mm, PEEK) was pumped with a mixture of mobile phases of 0.1% formic acid in water (mobile phase A) or 0.1% formic acid in acetonitrile (mobile phase B) (all reagents from Millipore Sigma). The column was equilibrated with 90% B for 8.3 min at 0.25 mL/min, after which 2.5 mL of extracted sample was injected. After this, a gradient ran from 90% to 20% B over 17.5, then was held at 20% B at 0.35 mL/min for 7.5 min. |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
Flow Gradient: | The column was equilibrated with 90% B for 8.3 min at 0.25 mL/min, after which 2.5 mL of extracted sample was injected. After this, a gradient ran from 90% to 20% B over 17.5 min, then was held at 20% B at 0.35 mL/min for 7.5 min |
Flow Rate: | 0.35 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |