Summary of Study ST002758
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001719. The data can be accessed directly via it's Project DOI: 10.21228/M8HF01 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002758 |
Study Title | Metabolic responses of normal rat kidneys to a high salt intake (Plasma) |
Study Type | Time-course metabolomics experiment |
Study Summary | In this study, novel methods were developed which allowed continuous (24/7) measurement of arterial blood pressure and renal blood flow in freely moving rats and the intermittent collection of arterial and renal venous blood to estimate kidney metabolic fluxes of O2 and metabolites. Specifically, the study determined the effects of a high salt (HS; 4.0% NaCl) diet upon whole kidney O2 consumption and arterial and renal venous plasma metabolomic profiles of normal Sprague-Dawley rats. A separate group of rats was studied to determine changes in the cortex and outer medulla tissue metabolomic profiles before and following the switch from a 0.4% to 4.0% NaCl diet. |
Institute | Medical College of Wisconsin |
Department | Physiology |
Laboratory | Dr. Allen W. Cowley |
Last Name | Cowley |
First Name | Allen |
Address | 8701 W. Watertown Plank Rd, Milwaukee, WI 53226 |
cowley@mcw.edu | |
Phone | 4149558277 |
Submit Date | 2023-06-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-02 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004475 | AN004476 | AN004477 | AN004478 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Area | Area | Area | Area |
Chromatography:
Chromatography ID: | CH003360 |
Chromatography Summary: | This chromatography method was utilized for all C18 positive polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 98% A1/2% B1, 1-13 minutes from 98% A1/2% B1 to 10% A1/90% B1, 13-15 minutes at 10% A1/90% B1, 15-16 minutes from 10% A1/90% B1 to 98% A1/2% B1, and was re-equilibrated from 16-25 minutes at 98% A1/2% B1 |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 100% water, 0.2% acetic acid |
Solvent B: | 100% acetonitrile, 0.2% acetic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003361 |
Chromatography Summary: | This chromatography method was utilized for all C18 negative polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 100% water, 0.2% acetic acid |
Solvent B: | 100% acetonitrile, 0.2% acetic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003362 |
Chromatography Summary: | This chromatography method was utilized for all HILIC positive polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent A2) |
Solvent B: | 90% ACN with 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent B2) |
Chromatography Type: | HILIC |
Chromatography ID: | CH003363 |
Chromatography Summary: | This chromatography method was utilized for all HILIC negative polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 10 mM ammonium acetate in H2O, pH 9.0 with 0.1% AffinityLab Deactivator Inhibitor (Agilent, #5191-3940; Solvent A3) |
Solvent B: | 85% ACN with 10 mM ammonium acetate in H2O with 0.1% AffinityLab Deactivator Inhibitor (Solvent B3) |
Chromatography Type: | HILIC |