Summary of Study ST003242

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003242
Study TitleLipidomic analysis of kidney from Gclc WT and whole-body Gclc KO mice.
Study SummaryWe found decreased triglycerides in the kidney of whole-body deletion of Gclc mice compared to wildtype mice.
Institute
University of Rochester Medical Center
Last NameHarris
First NameIsaac
Address601 Elmwood Ave, Rochester, New York, 14642-0001, USA
Emailisaac_harris@urmc.rochester.edu
Phone8572348624
Submit Date2024-05-29
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-06-07
Release Version1
Isaac Harris Isaac Harris
https://dx.doi.org/10.21228/M8DJ7S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005311 AN005312
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um) Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Normalized to the median value of total lipid signals Normalized to the median value of total lipid signals

Chromatography:

Chromatography ID:CH004016
Instrument Name:Thermo Vanquish
Column Name:Perkin Elmer Brownlee SPP C18 (75 × 2.1mm, 2.7um)
Column Temperature:50
Flow Gradient:0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B.
Flow Rate:0.400 mL/min
Solvent A:100% water; 0.1% formic acid; 10mM ammonium acetate
Solvent B:50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate
Chromatography Type:Reversed phase
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