Summary of Study ST003243
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003243 |
| Study Title | Lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
| Study Summary | The rate-limiting enzyme in glutathione (GSH) synthesis is Gclc. GSH synthesis is suggested to be implicated in lipogenesis. Loss of GSH synthesis is suggested to increase the activity of NRF2. To examine the impact of GSH and NRF2 on lipid abundance in serum, we performed lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
| Institute | University of Rochester Medical Center |
| Last Name | Harris |
| First Name | Isaac |
| Address | 601 Elmwood Ave, Rochester, New York, 14642-0001, USA |
| isaac_harris@urmc.rochester.edu | |
| Phone | 8572348624 |
| Submit Date | 2024-05-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005313 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalized to the median value of total lipid signals |
Chromatography:
| Chromatography ID: | CH004017 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B |
| Flow Rate: | 0.400 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 10mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate |
| Chromatography Type: | Reversed phase |
