Summary of Study ST003261
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002023. The data can be accessed directly via it's Project DOI: 10.21228/M8423Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003261 |
Study Title | Exploration of RSL3 or Chlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complex-induced changes in the phospholipid oxidation of MDA-MB-231 breast cancer cells |
Study Summary | Chlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complexes (SCs) exhibit potent anti-cancer properties through incompletely understood molecular mechanisms. Here, we treated human MDA-MB-231 triple-negative breast cancer cells with the glutathione peroxidase (GPX)4 inhibitor RSL3 or chlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complexes (SCs) and analyzed their oxidized phospholipid profile by targeted lipidomics. SCs induce extensive (hydroper)oxidation of arachidonic acid and adrenic acid in membrane phospholipids, particularly phosphatidylethanolamines (PE) and phosphatidylinositols (PC). In this process, SCs have demonstrated superior efficacy compared to the GPX4 inhibitor RSL3, an established ferroptosis inducer. Please note that one sample set was measured three times with the same sample-ID, but with different methods (oxPE, oxPC, oxPI), therefore each sub-class has their own raw-data file marked by their corresponding abbreviation (oxPE, oxPC, oxPI; e.g. "210309_MDA_Timecourse_RSL3_oxPE_dil_UD_Std_1ul_SFT.wiff", "210324_Rescue_Gust_compounds_oxPE_dil_UD_std_1ul_SFT.wiff", "210309_MDA_Timecourse_RSL3_oxPC_dil_UD_Std_1ul_SFT.wiff", "210324_Rescue_Gust_compounds_oxPC_dil_UD_std_1ul_SFT.wiff" or "210324_Rescue_Gust_compounds_oxPI_dil_UD_std_1ul_SFT.wiff", ). |
Institute | University of Innsbruck |
Last Name | Koeberle |
First Name | Andreas |
Address | Mitterweg 24, Innsbruck, Tyrol, 6020, Austria |
Andreas.Koeberle@uibk.ac.at | |
Phone | +43 512 507 57903 |
Submit Date | 2024-06-12 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2024-06-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005345 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity H-Class |
Column | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex 6500+ |
Ion Mode | NEGATIVE |
Units | absolute intensities |
Chromatography:
Chromatography ID: | CH004047 |
Chromatography Summary: | Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 130 Å, 2.1×100 mm, Waters, Milford, MA) using an ExionLC UHPLC system. |
Instrument Name: | Waters Acquity H-Class |
Column Name: | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
Column Temperature: | 45°C |
Flow Gradient: | The gradient was ramped from 75 to 85% B over 5 min and further increased to 100% B within 2 min, followed by isocratic elution for another 2 min. |
Flow Rate: | 0.75 ml/min |
Solvent A: | water/acetonitrile 90/10, 2 mM ammonium acetate |
Solvent B: | water/acetonitrile 5/95, 2 mM ammonium acetate |
Chromatography Type: | Reversed phase |