Summary of Study ST000286
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000228. The data can be accessed directly via it's Project DOI: 10.21228/M86014 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000286 |
Study Title | Mouse skeletal myotube chronic low-frequency stimulation |
Study Type | Electrical stimulation |
Study Summary | Cell culture media is collected from mouse myotubes subject to electrical stimulation and control cells with no stimulation. |
Institute | University of Florida |
Last Name | Vega |
First Name | Rick |
Address | -- |
rvega@sanfordburnham.org | |
Phone | -- |
Submit Date | 2015-02-10 |
Num Groups | 2 |
Total Subjects | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000454 | AN000455 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000: | Thermo Scientific-Dionex Ultimate 3000: |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH000324 |
Chromatography Comments: | UHPLC, Thermo Scientific-Dionex Ultimate 3000: While setting up sequence, ensure that method to be utilized is PFP-metabolomics-neg350-NoBuffer-0-SID and PFP-metabolomics-neg-350-dd-MS2 for negative sequences. For positive sequences utilize PFP-metabolomics-pos-350-0-SID and PFP-metabolomics-pos-350-dd-MS2. Check the lines for air bubbles and purge line if present. Set injection volume to 2uL for positive samples and 4uL for negative samples. |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000: |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |