Summary of Study ST000929
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000643. The data can be accessed directly via it's Project DOI: 10.21228/M8JT38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000929 |
Study Title | Metabolomics Analysis of Aqueous Humour in the Presence of Glaucoma Drainage Devices |
Study Type | Metabolomics Evaluation of Aqueous Humor Samples from Left (treated) and Right (Control) Eyes of Rabbits. |
Study Summary | Metabolomic experiments were carried out on aqueous humor samples provided by Ramesh Ayyala. Through this collaboration, we propose to study aqueous humor from control and experimental rabbits to better understand metabolic differences in the aqueous humor samples. |
Institute | University of North Carolina |
Department | Nutrition Research Institute |
Laboratory | ERCMRC |
Last Name | Sumner |
First Name | Susan |
Address | 500 Laureate Way, Kannapolis, NC, 28081, USA |
susan_sumner@unc.edu | |
Phone | 7042505066 |
Submit Date | 2018-02-11 |
Num Groups | 9 |
Total Subjects | 56 |
Num Males | 56 |
Analysis Type Detail | LC-MS |
Release Date | 2018-12-11 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001523 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt G2 Si QTOF |
Ion Mode | POSITIVE |
Units | Compound Normalised Abundance |
Chromatography:
Chromatography ID: | CH001073 |
Chromatography Summary: | Reversed-Phase Gradient Seperation |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 8 °C |
Flow Gradient: | Time(min) Flow Rate(mL/min) %A %B Curve 1. Initial 0.400 99.0 1.0 2. 1.00 0.400 99.0 1.0 3. 16.00 0.400 1.0 99.0 4. 20.00 0.400 1.0 99.0 5. 20.50 0.400 99.0 1.0 6. 22.00 0.400 99.0 1.0 |
Flow Rate: | 0.4 mL/min |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Weak Wash Solvent Name: | 10% MeOH |
Weak Wash Volume: | 1000 µL |
Strong Wash Solvent Name: | 80% MeOH |
Strong Wash Volume: | 1000 µL |
Target Sample Temperature: | 8 °C |
Sample Loop Size: | 10 µL |
Sample Syringe Size: | 100 µL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |