Summary of Study ST000973
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000646. The data can be accessed directly via it's Project DOI: 10.21228/M85M3S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000973 |
| Study Title | Metabolome profiles in urogenital schistosomiasis and associated pathologies (part II) |
| Study Summary | Metabolic fingerprint aid discovery of new biomarkers. Blood and urine were collected from volunteers in Eggua community, Nigeria, after ethical approval. LC-ESI-MS were used to analyse samples. Bioinformatics and statistical tools were used to detect important metabolites |
| Institute | University of Ibadan, Nigeria |
| Department | Zoology (Cell biology and Genetics unit) |
| Laboratory | Centre for Materials Characterization, National Chemical Laboratory, Pune, India |
| Last Name | Adewale |
| First Name | Adebayo |
| Address | Cell Biology and Genetics unit, Department of zoology, University of ibadan, Nigeria/ National Centre for Cell Science, Pune, India |
| walsaks@gmail.com | |
| Phone | 07030710368 |
| Submit Date | 2017-12-07 |
| Publications | doi:10.1371/journal.pntd.0006452 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001594 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column | Hypersil Gold |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | abundance of specific m/z (amu) |
Chromatography:
| Chromatography ID: | CH001119 |
| Chromatography Summary: | Both urine and plasma samples were prepared using chilled methanol:water mixture (4:1). Separation was achieved with 150mm x 2.1mm, 1.9μ Hypersil Gold column with a 5μl injection volume. Gradient elution was performed using 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase after optimization. The column was washed between each sample for stability and to eliminate carry-over. The flow rate was 0.35 ml/min. MS acquisition was performed on the Q-Exactive hybrid quadrupole orbitrap mass spectrometer (ThermoFisher, MA, USA) operated in positive and negative electrospray ionization (ESI) modes. The scan range was 70 to 1000 m/z. |
| Instrument Name: | Q-Exactive hybrid quadrupole orbitrap mass spectrometer |
| Column Name: | Hypersil Gold |
| Flow Rate: | 0.35ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
