Summary of Study ST001080
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000723. The data can be accessed directly via it's Project DOI: 10.21228/M87384 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001080 |
Study Title | Dynamic labeling of intracellular metabolites in PCC 7002 |
Study Type | Timecourse experiment |
Study Summary | The experiment was conducted in a reactor at a metabolic steady state (OD 730 nm = 0.8). 13C labeled sodium bicarbonate was used as a tracer. Tracer concentration was 1 g/L. After the introduction of the tracer, the samples were collected at time point : 0, 60, 120, 180 and 240 secs. Samples were quenched with methanol and extracted using methanol-chloroform-water method. Extracts were stored at -80 degree C till LCMS analysis. |
Institute | Indian Institute of Technology Bombay |
Department | Department of Chemical Engineering |
Laboratory | Bio systems Engineering Lab |
Last Name | Wangikar |
First Name | Pramod |
Address | Powai |
wangikar@iit.ac.in | |
Phone | 2225767232 |
Submit Date | 2018-10-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2019-08-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001763 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | Triple TOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001246 |
Instrument Name: | Shimadzu 20AD |
Column Name: | Phenomenex Synergi Hydro RP (100 x 2mm,2.5um) |
Flow Gradient: | 0% B (0 min), 0% B (2min), 35% B (8 min), 35% B (10.5 min), 90% B (15.50 min), 90% B (20.5 min) and 0% B (22 min) |
Flow Rate: | 0.3 mL/minute |
Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |