Summary of Study ST001262
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000847. The data can be accessed directly via it's Project DOI: 10.21228/M86H4F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001262 |
Study Title | The impact of tobacco smoke exposure and environmental exposures on the pulmonary microbiome of critically ill children |
Study Summary | This project evaluates the effects of tobacco smoke exposure (TSE) on the pediatric lung microbiome in critically ill children. The impact of TSE on the airway microbiome of critically ill, mechanically ventilated pediatric patients will be determined by through clinical outcomes and analysis of urinary and plasma metabolomes to identify other environmental exposures contributing to the alteration of the pediatric microbiome. |
Institute | Emory University |
Department | School of Medicine |
Laboratory | Clincal Biomarkers Laboratory |
Last Name | Uppal |
First Name | Karan |
Address | NA |
kuppal2@emory.edu | |
Phone | (404) 727 5027 |
Submit Date | 2019-06-25 |
Total Subjects | 367 |
Study Comments | Both CHEAR pooled urine samples and Clinical Biomarker Laboratory pooled plasma samples were used |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Chear Study | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2021-08-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002094 | AN002095 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | Reversed phase |
Chromatography system | Dionex UltiMate 3000 | Dionex UltiMate 3000 |
Column | Waters XBridge BEH Amide XP HILIC (50 x 2.1mm x 2.5um) with Thermo Accucore HILIC guard | Higgins endcapped C18 stainless steel (50 x 2.1mm,3um),Product #TS-0521-C183; Thermo Accucore C18 guard with holder,Product #17126-014005 |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH001529 |
Chromatography Summary: | The HILIC column is operated parallel to reverse phase column for simultaneous analytical separation and column flushing through the use of a dual head HPLC pump equipped with 10-port and 6-port switching valves. During operation of HILIC separation method, the MS is operated in positive ion mode and 10 microliters of sample is injected onto the HILIC column while the reverse phase column is flushing with wash solution. Flow rate is maintained at 0.35 mL/min until 1.5 min, increased to 0.4 mL/min at 4 min and held for 1 min. Solvent A is 100% LC-MS grade water, solvent B is 100% LC-MS grade acetonitrile and solvent C is 2% formic acid (v/v) in LC-MS grade water. Initial mobile phase conditions are 22.5% A, 75% B, 2.5% C hold for 1.5 min, with linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min, resulting in a total analytical run time of 5 min. During the flushing phase (reverse phase analytical separation), the HILIC column is equilibrated with a wash solution of 77.5% A, 20% B, 2.5% C. |
Methods ID: | 2% formic acid in LC-MS grade water |
Methods Filename: | 20160920_posHILIC120kres5min_ESI_c18negwash.meth |
Chromatography Comments: | Triplicate injections for each chromatography mode |
Instrument Name: | Dionex UltiMate 3000 |
Column Name: | Waters XBridge BEH Amide XP HILIC (50 x 2.1mm x 2.5um) with Thermo Accucore HILIC guard |
Column Temperature: | 60C |
Sample Injection: | 10 uL |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Analytical Time: | 5 min |
Sample Loop Size: | 15 uL |
Sample Syringe Size: | 100 uL |
Chromatography Type: | HILIC |
Chromatography ID: | CH001530 |
Chromatography Summary: | The C18 column is operated parallel to the HILIC column for simultaneous analytical separation and column flushing through the use of a dual head HPLC pump equipped with 10-port and 6- port switching valves. During operation of the "C18 method, the MS is operated in negative ion mode and 10 μL of sample is" injected onto the C18 column while the HILIC column is flushing with wash "solution. Flow rate is maintained at 0.4 mL/min until 1.5 min, increased to 0.5" "mL/min at 2 min and held for 3 min. Solvent A is 100% LC-MS grade water, solvent" B is 100% LC-MS grade acetonitrile and solvent C is 10mM ammonium acetate in "LC-MS grade water. Initial mobile phase conditions are 60% A, 35% B, 5% C hold" "for 0.5 min, with linear gradient to 0% A, 95% B, 5% C at 1.5 min, hold for 3.5" "min, resulting in a total analytical run time of 5 min. During the flushing" "phase (HILIC analytical separation), the C18 column is equilibrated with a wash" "solution of 0% A, 95% B, 5% C until 2.5 min, followed by an equilibration" "solution of 60% A, 35% B, 5% C for 2.5 min." |
Methods ID: | 10mM ammonium acetate in LC-MS grade water |
Methods Filename: | 20160920_negC18120kres5min_ESI_HILICposwash.meth |
Instrument Name: | Dionex UltiMate 3000 |
Column Name: | Higgins endcapped C18 stainless steel (50 x 2.1mm,3um),Product #TS-0521-C183; Thermo Accucore C18 guard with holder,Product #17126-014005 |
Column Temperature: | 60C |
Flow Rate: | 0.4 mL/min for 1.5 min; linear increase to 0.5 mL/min at 2 min held for 3 min |
Sample Injection: | 10 uL |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Analytical Time: | 5 min |
Sample Loop Size: | 15 uL |
Sample Syringe Size: | 100 uL |
Chromatography Type: | Reversed phase |