Summary of Study ST001514
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001021. The data can be accessed directly via it's Project DOI: 10.21228/M8QM5H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001514 |
Study Title | Community metabolomes reflect taxon-specific fingerprints of phytoplankton in the ocean |
Study Type | Metabolomic survey of 21 phytoplankton species |
Study Summary | Phytoplankton transform inorganic carbon into thousands of biomolecules, including polar metabolites that represent an important pool of labile fixed carbon, nitrogen, and sulfur. Metabolite production is not identical among phytoplankton, and the flux of these molecules through the microbial loop depends on compound-specific bioavailability to a wider microbial community. Yet relatively little is known about the diversity or concentration of polar metabolites within marine plankton. Here we evaluate 313 metabolites in 21 phytoplankton species and in natural marine particles across environmental gradients to show that bulk community metabolomes reflect the phytoplankton community on a chemical level. |
Institute | University of Washington |
Department | Oceanography |
Laboratory | Ingalls Lab |
Last Name | Heal |
First Name | Katherine |
Address | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA, 98195, USA |
kheal@uw.edu | |
Phone | 612-616-4840 |
Submit Date | 2020-10-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-01-25 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002508 | AN002509 | AN002510 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | HILIC | HILIC |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
Column | Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
Units | Adjusted and normalized peak areas | Adjusted and normalized peak areas | Adjusted and normalized peak areas |
Chromatography:
Chromatography ID: | CH001833 |
Chromatography Summary: | See attached summary. |
Methods Filename: | Ingalls_Lab_LC_Methods.txt |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um) |
Column Temperature: | 35 |
Flow Rate: | 0.4 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001834 |
Chromatography Summary: | See attached summary |
Methods Filename: | Ingalls_Lab_LC_Methods.txt |
Instrument Name: | Waters Acquity I-Class |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 30 |
Flow Rate: | 0.15 ml/min |
Solvent A: | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
Solvent B: | 15% acetonitrile/85% water; 10 mM ammonium carbonate |
Chromatography Type: | HILIC |