Summary of Study ST001990
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001264. The data can be accessed directly via it's Project DOI: 10.21228/M8B39F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001990 |
Study Title | Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: mechanisms of attack and survival |
Study Type | Untargeted Metabolomics |
Study Summary | One of the most concerning pests that attack strawberries in Brazil is Duponchelia fovealis, a non-native moth with no registered control methods to date. Our group recently observed that a fungal consortium formed by two strains of Beauveria bassiana increased the mortality of D. fovealis more than inoculation with each strain on its own. However, the molecular interaction between the fungal consortium and the caterpillars is unknown, raising several questions about the enhanced pest control observed. Furthermore, concerns over the emergency of resistance and the selection for resistance to chemical and biological products that are constantly applied in agriculture highlight the need for careful examination of novel pest control methods. Thus, in this work, we sought to pioneer the evaluation of the molecular interaction between a fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to understand the biocontrol process involved in this interaction and the defense system of the caterpillar. Therefore, seven days after D. fovealis caterpillars were inoculated with the B. bassiana consortium, the dead and surviving caterpillars were analyzed using GC-MS and LC-MS/MS. |
Institute | Universidade Federal do Paraná |
Department | Patologia Básica |
Laboratory | Laboratório de Microbiologia e Biologia Molecular |
Last Name | Katiski da Costa Stuart |
First Name | Andressa |
Address | Av. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, Brazil |
andressa.katiski@gmail.com | |
Phone | 55 41 991922779 |
Submit Date | 2021-11-12 |
Num Groups | 7 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf, raw(Waters) |
Analysis Type Detail | API-MS |
Release Date | 2023-05-12 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003242 | AN003243 | AN003244 | AN003245 | AN003246 | AN003247 |
---|---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS | MS |
Chromatography type | GC | GC | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 7890A | Agilent 7890A | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC |
Column | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
MS Type | EI | API | ESI | ESI | ESI | ESI |
MS instrument type | GC x GC-TOF | GC x GC-TOF | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF | Leco Pegasus 4D GCxGC TOF | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC |
Ion Mode | UNSPECIFIED | UNSPECIFIED | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
Units | peak area | Relative intensity | Relative intensity | Relative intensity | Relative intensity |
Chromatography:
Chromatography ID: | CH002391 |
Methods Filename: | Metabolomics_Methods |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) |
Column Temperature: | 70 - 320 ºC |
Flow Rate: | 1 mL.min-1 |
Injection Temperature: | 280 ºC |
Sample Injection: | 1 uL |
Oven Temperature: | 70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then held at this temperature for 4 min. |
Chromatography Type: | GC |
Chromatography ID: | CH002392 |
Methods Filename: | Metabolomics_Methods |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
Column Temperature: | 35 ºC |
Flow Gradient: | 95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min |
Flow Rate: | 0.5 mL·min-1 |
Solvent A: | Water; formic acid |
Solvent B: | 100% acetonitrile; formic acid. |
Capillary Voltage: | 3 kV |
Chromatography Type: | Reversed phase |