Summary of Study ST002183
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001390. The data can be accessed directly via it's Project DOI: 10.21228/M82117 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002183 |
| Study Title | Individualized exercise intervention for people with multiple myeloma improves quality of life in a randomized controlled trial |
| Study Summary | Although new treatments have improved survival for multiple myeloma (MM), quality of life remains poor for people with this incurable cancer. We conducted a multi-site randomized, waitlist-controlled trial of an individualized exercise program for people at all stages of MM (n=60). Compared to the waitlist control group, participants of the 12-week intervention had significant improvement in health-related quality of life, mediated through improved MM symptoms, cardiorespiratory fitness and bone pain, with were mostly maintained at follow-up (up to 12 months). Exploratory plasma metabolomics and lipidomics was conducted to delineate molecular mechanisms and biomarkers |
| Institute | QIMR Berghofer Medical Research Institute |
| Laboratory | Precision & Systems Biomedicine |
| Last Name | Stoll |
| First Name | Thomas |
| Address | 300 Herston Road |
| thomas.stoll@qimrberghofer.edu.au | |
| Phone | +61 7 3845 3992 |
| Submit Date | 2022-06-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003575 | AN003576 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm | Agilent Poroshell 120 HILIC-Z (100 2.1mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002643 |
| Chromatography Summary: | Reversed-phase separation of metabolites was adapted from Evans et al. (2014) with modifications. Separation was achieved on a Zorbax Eclipse Plus C18 RRHD (1.8 μm, 95 Å, 2.1x50mm, #959757-902, Agilent) column held at 40°C and the MS was operated in positive ionization mode. Eluent A was milliQ water and eluent B was methanol both containing 0.1% formic acid. Total method runtime was 8.5 min with the following gradient: 0 min (0.5% eluent B) – 4 min (70% B) – 4.5 min (98% B) – 5.4 min (98% B) – 5.5 min (0.5% B) – 8.5 min (0.5% B). The flow was diverted to waste after 5 minutes and a flow rate of 0.4 mL/min was applied |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min (0.5% eluent B) - 4 min (70% B) - 4.5 min (98% B) - 5.4 min (98% B) - 5.5 min (0.5% B) - 8.5 min (0.5% B). The flow was diverted to waste after 5 minutes |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002644 |
| Chromatography Summary: | HILIC separation of metabolites was carried out on a Poroshell 120 HILIC-Z (100Å, 2.7µm, 2.1x100mm, #675775-924, Agilent) column at 30ºC and the MS was operated in negative ionization mode. Eluent A was 10 mM ammonium acetate and 5 μM medronic acid in ACN/milliQ 90:10 (pH 9) and eluent B was 10 mM ammonium acetate and 5 μM medronic acid in milliQ (pH 9). Gradient applied was: 0 min (10% eluent B) – 3.5 min (25% B) – 5.5 min (50% B) – 7.5 min (50% B) – 7.6 min (10% B) – 14 min (10% B). Flow rate was set to 0.25 mL/min with a flow ramp to 0.5 mL/min during equilibration |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Poroshell 120 HILIC-Z (100 2.1mm,2.7um) |
| Flow Gradient: | Gradient applied was: 0 min (10% eluent B) - 3.5 min (25% B) - 5.5 min (50% B) - 7.5 min (50% B) - 7.6 min (10% B) - 14 min (10% B). |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 90% acetonitrile/10% water; 10 mM ammonium acetate; 5 µM medronic acid, pH 9 |
| Solvent B: | 100% water; 10 mM ammonium acetate; 5 µM medronic acid, pH 9 |
| Chromatography Type: | HILIC |
