Summary of Study ST002186
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001393. The data can be accessed directly via it's Project DOI: 10.21228/M8NT4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002186 |
Study Title | An early-life microbiota metabolite protects against obesity via intestinal PPAR-gamma |
Study Type | untargeted metabolomics analysis |
Study Summary | The mechanisms by which the early-life microbiota protects against environmental factors that promote childhood obesity remain largely unknown. Using a mouse model in which young mice are simultaneously exposed to antibiotics and a high-fat diet, we show that Lactobacillus species, predominant members of the small intestine microbiota, regulate intestinal epithelial cells (IECs) to limit diet-induced obesity during early-life. A Lactobacillus-derived metabolite, phenyllactic acid (PLA), protected against metabolic dysfunction caused by early-life exposure to antibiotics and a high-fat diet by increasing the abundance of peroxisome proliferator activated receptor gamma (PPAR-gamma) in the small intestine IECs. Therefore, PLA is a microbiota-derived metabolite that activates protective pathways in the small intestine epithelium to regulate fat absorption and prevent obesity during early life. |
Institute | Vanderbilt University |
Department | Chemistry |
Laboratory | Center for Innovative Technology |
Last Name | Codreanu |
First Name | Simona Gabriella |
Address | 1234 Stevenson Center Lane |
SIMONA.CODREANU@VANDERBILT.EDU | |
Phone | 6158758422 |
Submit Date | 2022-06-03 |
Num Groups | 4 |
Total Subjects | 20 |
Num Males | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-06-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003580 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | time_m/z |
Chromatography:
Chromatography ID: | CH002647 |
Chromatography Summary: | Prepared samples were analyzed by RPLC-HRMS/MS in the Vanderbilt Center for Innovative Technology (CIT) using a modified version of a reversed phase chromatography negative ionization method. Metabolites were separated on a Thermo Fisher Scientific (Waltham, MA) Hypersil Gold C18 column (100 x 2.1 mm, 1.9 μm particle size) using water/acetonitrile gradient with formic acid (0.1%) added to both mobile phases. |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Hypersil Gold (100 x 2. mm,1.9um) |
Column Temperature: | 40 |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 80% acetonitrile/20% water; 0.1% formic acid |
Chromatography Type: | Reversed phase |