Summary of Study ST002514
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001622. The data can be accessed directly via it's Project DOI: 10.21228/M81T5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002514 |
| Study Title | The investigation of the role of dietary inulin in NASH progression with mouse fecal metabolites |
| Study Type | non-targeted LC-Mass analysis |
| Study Summary | To investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Additionally, some mice received inulin or cellulose was treated with 13C labelled fiber for 36 hours. Fecal metabolites were analyzed by non-targeted metabolomics.Results shown that the fecal metabolites from Inulin treatment group signficantly distinguished from that from CDHFD only group, while only negligible alterations was induced by cellulose treatment, indicating that inulin not cellulose could be well fermented by gut microbiota. The 13C tracing results shown that nineteen 13C-inulin labelled metabolites were also captured including pantothenate, phosphoethanolamine and adenosine. These metabolites are reported to play a protective role in reducing fat accumulation and ameliorating cellular oxidative stress and inflammation, indicating that the mechanism inulin suppresses NASH may through mediating modulating gut metabolites. |
| Institute | The Chinese University of Hong Kong |
| Last Name | Wei |
| First Name | Wenchao |
| Address | Prince of Wales Hospital |
| 1155118538@link.cuhk.edu.hk | |
| Phone | 56001913 |
| Submit Date | 2023-03-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004140 | AN004141 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak ion intensity | Peak ion intensity |
Chromatography:
| Chromatography ID: | CH003066 |
| Chromatography Summary: | Non-targeted metabolomic analysis was performed on a 1290 Infinity series UHPLC System (Agilent Technologies) coupled to a Triple TOF 6600 mass spectrometer (AB Sciex ) equipped with an electrospray ionization (ESI) source by Biotree company (Shanghai, China). Briefly, chromatographic separation was achieved by using a Waters BEH Amide column (2.1 * 100 mm, 1.7 µm) under 25 ℃. The mobile phase consisted of water with 25mM ammonium acetate and 25 mM ammonium hydroxide (A) and acetonitrile (B). The flow rate was 0.5 ml/min. |
| Methods Filename: | Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Column Temperature: | 25 ℃ |
| Flow Gradient: | 0~0.5 min, 95% B; 0.5~7 min, 95%~65% B; 7~8 min, 65%~40% B; 8~9 min, 40% B; 9~9.1 min, 40%~95% B; 9.1~12 min, 95% B |
| Flow Rate: | 0.5 ml/min. |
| Solvent A: | water with 25mM ammonium acetate and 25 mM ammonium hydroxide |
| Solvent B: | acetonitrile |
| Chromatography Type: | HILIC |
