Summary of Study ST003009
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003009 |
Study Title | Media_15N BCAA tracing in brown adipocyte |
Study Summary | To determine the metabolic fate and nitrogen flux of BCAA in mouse brown adipocytes, we used 15N labeled BCAA tracing (Leu (NLM-142-1, CIL), Ile (NLM-292-0.25, CIL) and Val (NLM-316-0.5, CIL)) followed by LC-MS analysis. |
Institute | Harvard Medical School |
Last Name | Wang |
First Name | Dandan |
Address | 3 Blackfan Circle |
dandanwang2022@gmail.com | |
Phone | 5083733714 |
Submit Date | 2023-12-13 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-02-19 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004940 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Vanquish Horizon |
Column | Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo orbitrap exploris 240 |
Ion Mode | NEGATIVE |
Units | Labeled peak areas (normalized) |
Chromatography:
Chromatography ID: | CH003729 |
Instrument Name: | Vanquish Horizon |
Column Name: | Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um) |
Column Temperature: | 25 °C |
Flow Gradient: | The linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 25mM ammonium acetate; 25mM ammonium hydroxide |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |