Summary of Study ST003071
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001913. The data can be accessed directly via it's Project DOI: 10.21228/M8FH9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003071 |
| Study Title | Attenuation of Helicobacter pylori VacA toxin-induced cell death by modulation of intracellular taurine metabolism - Study #4 |
| Study Type | untargeted metabolomics analysis |
| Study Summary | Repletion of intracellular taurine is sufficient to modulate VacA-induced cell death. To determine how supplemental taurine influences cellular metabolism to prevent VacA-induced cell death, we treated both AGS and AZ-521 cells with acid-activated VacA or an acidified buffer control for 12 hours or 5 hours, respectively, in the presence or absence of 10 mM extracellular taurine. Following intoxication, untargeted global metabolomics was conducted on the cell pellets. When the metabolic profiles of VacA-treated cells in the absence of extracellular taurine were compared to the metabolic profiles of VacA-treated cells in the presence of extracellular taurine, taurine was the only metabolite exhibiting significantly different levels in AZ-521 cells and one of two metabolites altered in AGS cells. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | CODREANU |
| First Name | SIMONA |
| Address | 1234 STEVENSON CENTER LANE |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2024-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005029 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | time_m/z |
Chromatography:
| Chromatography ID: | CH003800 |
| Methods Filename: | MS_Methods_Cover_Taurine.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) |
| Column Temperature: | 40 |
| Flow Gradient: | 30 min; 95%A, 5%B |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water, 0.1% Formic Acid |
| Solvent B: | 80:20 acetonitrile:water, 0.1% Formic Acid |
| Chromatography Type: | Reversed phase |
