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MB Sample ID: SA002823
Local Sample ID: | MR2_AZD_253 |
Subject ID: | SU000074 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | FLT3-ITD AML (MR2) |
Cell Biosource Or Supplier: | Dr. Tim Pardee (Wake Forest Univ.) |
Cell Primary Immortalized: | Primary |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO000057 |
Collection Summary: | Conditioned Medias:Cellular Fraction_LC-MS:Cellular Fraction_WB |
Collection Protocol Comments: | Processed simultaneously/time point:Processed simultaneously/time point:Processed simultaneously/time point |
Sample Type: | conditioned treatment media |
Collection Method: | Total contents pipette-transferred to tubes & centrifuged at 1800 rpm for 5 minutes. Medias transferred into aliquot tubes.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vials based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vial based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage. |
Collection Location: | RTI RCMRC Tissue culture suite:RTI RCMRC Wet lab:RTI RCMRC Wet lab |
Collection Frequency: | 0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h |
Collection Duration: | 10 minutes:10 minutes:5 minutes |
Collection Time: | 0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h |
Volumeoramount Collected: | 1 mL x 5 aliquots:2x10^7 cells:2x10^6 cells |
Storage Conditions: | -80C:-80C:-80C |
Collection Vials: | 15 mL conical tubes:15 mL conical tubes:15 mL conical tubes |
Storage Vials: | 1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes |
Collection Tube Temp: | chilled in ice:chilled in ice:chilled in ice |