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MB Sample ID: SA008997

Local Sample ID:86
Subject ID:SU000184
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Sprague–Dawley
Gender:female
Species Group:Mammal

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Collection:

Collection ID:CO000170
Collection Summary:The tibialis anterior (TA), extensor digitorum longus (EDL), plantaris, gastrocnemius and soleus muscles were dissected from the loaded left leg and the unloaded right leg immediately after death. One half of the soleus and EDL muscles together with TA and gastrocnemius were quickly frozen in liquid propane cooled by liquid nitrogen, and stored at ?160°C for further analyses. In the other halves of the soleus and EDL muscles, bundles of approximately 50 fibres were dissected from the muscles in relaxing solution at 4°C and tied to glass capillaries, stretched to about 110% of their resting slack length. The bundles were chemically skinned for 24 h in relaxing solution containing 50% (v/v) glycerol for 24 h at 4°C and were subsequently stored at ?20°C (Larsson & Moss, 1993). All the bundles were cryo-protected within 1 week after skinning by transferring the bundles every 30 min to relax solution containing increasing concentrations of sucrose, i.e. 0, 0.5, 1.0, 1.5 and 2.0 m, and subsequently frozen in liquid propane chilled with liquid nitrogen (Frontera & Larsson, 1997). The frozen bundles were stored at ?160°C pending use. One day before the experiments, a bundle was transferred to a 2.0 m sucrose solution for 30 min, subsequently incubated in solutions of decreasing sucrose concentration (1.5–0.5 m) and finally kept in a skinning solution at ?20°C.
Sample Type:Muscle
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