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MB Sample ID: SA009098
Local Sample ID: | 36 |
Subject ID: | SU000186 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | SpragueDawley rats |
Weight Or Weight Range: | 300400 g |
Species Group: | Mammal |
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Collection:
Collection ID: | CO000172 |
Collection Summary: | Hepatocytes were isolated from male SpragueDawley rats (300400 g; Harlan, Indianapolis, IN, USA) by a two-step perfusion method as previously described (33). All harvests yielded hepatocytes with viability exceeding 95% by trypan blue dye exclusion. Freshly isolated hepatocytes were suspended in SFM, composed of Williams E supplemented with 0.2 U/ml insulin, 6 ?g/ml transferrin, 100 U/ml penicillin G, 100 mg/ml streptomycin, 3 g/L human albumin, 2.2 g/L sodium bicarbonate, 1 g/L L-carnitine, 2.0 mM L-glutamine, 100 nM dexamethasone, 40 ng/ml glucagon, 20 ng/ml Gly-His-Lys, 1,000 U/L heparin, 1 mg/L warfarin, and 5 ng/ml of mouse epidermal growth factor (EGF) (3). The cells, suspended in SFM, were placed in a spheroid box (33 × 28 × 6 cm) custom-made of polycarbonate by Mayo Division of Engineering and siliconized with Sigmacote for 30 min (20) and gently rocked continuously at a frequency of 10 cycles per minute (0.17 Hz) to induce spheroid formation and to maintain spheroids in suspension. Final hepatocyte concentration was 5 × 105 cells/ml per spheroid box. All culture conditions were maintained in a 5% CO2, 37°C incubator as previously described (20). Spheroids were centrifuged and resuspended in fresh culture media every 24 h. |
Sample Type: | Liver |